Quorum Technologies
Light at Work
Confocal NL RCM2
Sharp images, a large field of view and easy to use? It’s possible with Confocal.nl. Get more from your samples, with a higher resolution and a higher sensitivity than conventional confocal microscopes and study live samples longer.
The Confocal NL Re-scanning Confocal Microscope (RCM) uses multiple laser pointers and a camera as a detector. The low laser power required is live cell friendly: it prevents phototoxicity in your live samples and photobleaching of your fluorophores. Creating long-term time lapses at a super-resolution was never this accessible.
ReScan Confocal: RCM2
RCM2 is our second generation RCM, with digital scanner technology. It makes bi-directional scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!
Improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.
The benefits of RCM2
Using RCM2 with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.
Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.
Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.
Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.
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Discover super-resolution imaging with RCM2
Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions.
The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).
In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples
