X-Light V2 Confocal System
The X-Light V2 is a high-performance confocal imaging system ideally suited for both core imaging facilities and individual research labs. This spinning disk confocal system can seamlessly switch between widefield, confocal, and Super-resolution imaging (with the optional VCS add-on) on the same microscope.
The X-Light V2 comes standard with a 5-position motorized dichroic wheel, 8-position motorized emission filter wheel, motorized bypass mode and exchangeable disk boxes. An 8 position motorized excitation wheel is an available option. The X-Light V2 has has a large field of view it works with both EMCCDs, for the sensitive and low-light applications, as well as larger-format sCMOS cameras for higher resolution imaging. The X-Light V2 is compatible with the new high power, low cost LDI laser illuminator.
From more information please contact: This email address is being protected from spambots. You need JavaScript enabled to view it.
Mulit Modal
- A rapid motorized Disk In/DiskOut control provides access to widefield and confocal imaging modes. Switching between modes is automated by imaging software.
Nipkow Spinning Disk
- A proprietary disk pattern design provides high confocal resolution, improved out‐of‐focus rejection and higher ratio of signal‐to‐noise. The result is < 800 nm confocal resolution with 60x 1.4 N.A. oil immersion objective.
Disks are available with pinholes sized for best results from different objectives:
- 40–μm holes, for objectives with N.A. < 1;
- 70–μm holes, for objectives with N.A. > 1;
- Custom pinhole diameters are available.
Disks are available to best suit particular camera setups.
- Single Hole Pattern: 22×22–μm field of view for large chips of scientific CMOS cameras;
- Dual Hole Patterns: 12×12–μm field of view for each pattern, for flexibility for smaller chips of CCD cameras.
Motor
- The disk rotates at 15,000 revolutions per minute, providing excitation and imaging at speeds of current cameras.
Illumination Subsystems
- The excitation mount accomodates LED light sources or multimode fiber for lasers; its SMA connector provides high‐efficiency coupling;
- The gimbal mount for excitation eases alignment to produce the highest ratio of signal to noise;
- The motorized five‐position dichroic filter wheel and eight‐position emission filter wheel provide for automation and ease of interactive control.
VCS (VideoConfocal super-resolution) module is a new system developed as an add-on for the X-light V2.
Using an approach based on super-resolution patents from Pisa CNR (which assume the 2-dimensional scan of the sample with a pinhole pattern and several algorithms application), CrestOptics-VCS enhance 3D resolution of the standard light microscope.
Parallel calculation is implemented exploiting CUDA technology for a very fast processing time.
The full system X-Light V2 + VCS configurations:
- Widefield mode
- Confocal mode
- Super-resolution mode
- Live cell confocal imaging
- 3D optical sectioning
- Simultaneous multi-channel imaging (with optional emission splitting systems)
- Super-resolution imaging with optional VCS add-on
