Dr. Brenda Andrews, February 2010
We use a Quorum WaveFX spinning disc confocal microscope to track fluorescently tagged proteins in the budding yeast Saccharomyces cerevisiae. Using the WaveFX system, we can accurately determine the sub-cellular distributions of tagged proteins expressed from their endogenous chromosomal loci in live cells and track changes in these distributions over time. The quality of the images acquired on this microscopy platform far exceeds that which we have obtained using our previous microscope or other systems we have encountered in other laboratories. The WaveFX system has an intuitive and robust design, which has enabled us to quickly teach the skills necessary for its operation to new users. We are also extremely satisfied with the fast and efficient technical support we receive from Quorum and highly recommend it to others. |