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Structured Illumination

Structured light illumination is a recently developed technique and is used to obtain high resolution optical sections of cells and tissues.    A practical alternative to high end laser confocal systems, structured illumination provides a high throughput, cost effective, easy to use solution. 

The angstrom™ was developed by Quorum Technologies to optimize image quality using structured light.  This involves the projection of a grid structure onto the focal plane of the sample.  Although many microscopes are capable of using structured light illumination, few can do this effectively.  Quorum has successfully improved the optical system using chromatically correct, enhanced optics which allow for the best possible grid projection.

The angstrom™ replaces the traditional fluorescence light path with an external light pathway.  The result is a revolutionary advance in how routine fluorescence imaging is performed.

Quorum Angstrom™

Angstrom™ is a desktop system with single body construction that can be installed on any brightfield or fluorescence microscope. To upgrade your existing microscope stand, simply attach the angstrom™ to the video-port on the side of your microscope.  Extremely portable the angstrom™ can be moved easily from one microscope to another.

Download Data Sheet

image

Images captured using Quorum angstrom™
Courtesy of The Human Protein Atlas and
Barbe L, Lundberg E, Oksvold P, Stenius A, Lewin E, Bjorling E, Asplund A, Ponten F, Brismar H, Uhlen M and Andersson-Svahn H. "Towards a confocal subcellular atlas of the human proteome." Mol Cell Proteomics 2007 Nov 19
Key Features
  • Generates confocal quality images without lasers
  • Automatic grid calibration and focusing
  • Auto-stitching/tiling of images
  • Reduces photobleaching
  • Suitable for fixed or live cell imaging
  • Large spectral flexibility which allows for multiple or uncommon fluorochromes
  • Not necessary to switch grids when changing objectives
  • The angstrom can be easily removed, allowing for standard widefield imaging
  • Parfocality at all wavelengths, including UV and visible

 

Specifications

Images

 
 
 
   
 

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